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Objective:To study the macrolides resistance of Mycoplasma pneumoniae(MP) in Suzhou area, and try to explore the relationship between drug resistance and refractory Mycoplasma pneumoniae pneumonia (RMPP). Methods:From a series of hospitalized children who were diagnosed as Mycoplasma pneumoniae pneumonia (MPP) from October 2013 to September 2014 in Suzhou area, 48 children were treated with Azithromycin (10 mg/kg, once a day, intravenous drip for 5-7 days), and the clinical symptoms and chest imaging were still progressing so they were clinically diagnosed as RMPP, and 34 children who were successfully treated with macrolides antibiotics (MA) were clinically diagnosed as general MPP (GMPP). MP DNA was extracted from the airway secretion of children in the two groups, and the point mutations of 2063 and 2064 of 23S rRNA were sequenced, and according to the MP 23S rRNA sequencing results, the children were divided into macrolides antibiotic resistant MP group (MRMP) and macrolides antibiotic sensitive MP group (MSMP). The clinical characteristics of the two groups were compared. Results:In the MRMP group, the incidence of RMPP was 62.2% (46/74 cases), while in MSMP group, the incidence of RMPP was 25.0% (2/8 cases). The point mutation of MP 23S rRNA had no significant effect on the occurrence of RMPP ( χ2=2.719, P=0.099). Compared with MRMP group, MSMP group presented shorter fever time and less glucocorticoid use.No significant differences between the two groups were found in chest imaging examination, as well as some laboratory results, including the total number and classification of white blood cell (WBC), C-reactive protein (CRP), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) and creatine kinase isoenzyme (CK-MB). Conclusions:The fever duration of MPP lasted more than 1 week, suggesting the possibility of macrolides resistance of MP, but macrolides resistance did not aggravate the occurrence of RMPP.It is unreliable to judge the MRMP by chest imaging features and laboratory results.
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Objective:To explore the effect of bifidobacterium tetravaccine tablets combined with low-dose erythromycin on the efficacy and motilin (MTL) levels in the treatment of children with functional dyspepsia (FD).Methods:One hundred and fifty children with FD admitted to Shanghai Public Health Clinical Center from January 2019 to June 2020 were selected and were divided into two groups by random number table, with 75 cases in each group. The control group was treated with bifidobacterium tetravaccine tablets, and the observation group was treated with low-dose erythromycin on the basis of control group. The clinical efficacy and the disappearance time of the clinical symptoms of the two groups were compared. The changes in the frequency of gastric antrum contraction (ACF), the amplitude of gastric antrum contraction (ACA), the gastric antral movement index (MI) were detected by ultrasonic developing-out unit before and after treatment. The levels of 5-hydroxytryptamine (5-HT), nitric oxide (NO), gastrin (GAS) and MTL were detected before and after the treatment using enzyme linked immunosorbent assay. The incidence of adverse reactions was counted. The recurrence rate was recorded after 6 months of the treatment.Results:The total effective rate in the observation group was higher than that in the control group:93.33% (70/75) vs. 78.67% (59/75), and the difference was statistically significant ( χ2 = 6.700, P<0.05). The disappearance time of symptoms of nausea and vomiting, loss of appetite, abdominal distension, and abdominal pain in the observation group were shorter than those in the control group: (2.58 ± 0.45) d vs. (3.22 ± 0.68) d, (3.56 ± 0.62) d vs. (4.10 ± 0.70) d, (2.54 ± 0.46) d vs. (3.24 ± 0.69) d, (2.66 ± 0.56) d vs. (3.40 ± 0.67) d, and the differences were statistically significant ( P<0.05). The levels of ACF, ACA and MI in the observation group after the treatment were higher than those in the control group: (4.32 ± 0.90) times/min vs. (3.58 ± 0.83) times/min, (0.34 ± 0.12) vs. (0.30 ± 0.10) mm, (1.33 ± 0.42) vs. (1.14 ± 0.30), and the differences were statistically significant ( P<0.05). The levels of 5-HT and NO in the observation group after the treatment were lower than those in the control group: (164.77 ± 30.34) mg/L vs. (184.45 ± 28.90) mg/L, (38.27 ± 10.20) mmol/L vs. (46.54 ± 9.48) mmol/L; and the levels of GAS and MTL were higher than those in the control group: (115.23 ± 15.70) ng/L vs. (98.50 ± 14.29) ng/L, (266.67 ± 32.76) ng/L vs. (238.40 ± 28.97) ng/L, and the differences were statistically significant ( P<0.05). Conclusions:The treatment of pediatric FD with bifidobacterium tetravaccine tablets combined with low-dose erythromycin can significantly improve the efficacy, quickly improve the clinical symptoms of children, promote physical recovery, and regulate MTL levels to improve gastric motility. The recurrence rate is low.
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Objective@#To observe the effect of different expression levels of USP28 on the radiosensitivity of ECA109 cells by gene transfection method, aiming to provide theoretical basis for comprehensive treatment of esophageal cancer.@*Methods@#The expression levels of USP28 and c-Myc in the esophageal epithelial cells Het-1A, ECA109 and ECA109R were quantitatively measured by qRT-PCR. The specific siRNA sequences were designed according to the USP28 and c-Myc genes. The pcDNA-USP28 and pcDNA-c-Myc plasmids were constructed. The esophageal cancer cell ECA109 was transfected with Lipofectamine 2000 to observe the transfection effect and related protein expression. ECA109 and ECA109R cells were exposed to 6 Gy X-ray radiation. The cell apoptosis in each group was detected by flow cytometry. The radiosensitivity was evaluated by clone formation assay.@*Results@#The expression levels of USP28 and c-Myc in ECA109 were significantly higher than those in Het-1A (both P<0.05), and the expression levels of USP28 and c-Myc in ECA109R were remarkably higher than those in ECA109(both P<0.05). The pcDNA-USP28 and pcDNA-c-Myc recombinant plasmids were successfully constructed. Compared with the negative control group, the expression of USP28 at the protein and mRNA levels in the si-USP28 group was significantly down-regulated, whereas those in the pcDNA-USP28 group were remarkably up-regulated. Similar results were obtained in terms of c-Myc. Compared with the control group, the expression level of c-Myc protein was significantly up-regulated in the pcDNA-USP28 group, whereas considerably down-regulated in the si-USP28 group. After 6 Gy irradiation, the apoptosis rate and radiosensitivity of ECA109 cells were significantly declined. The apoptosis rate and radiosensitivity of ECA109R cells were increased in the si-USP28 group.@*Conclusions@#The expression of USP28 protein is closely correlated with the radiosensitivity of esophageal cancer cells. The underlying mechanism may be related to the regulation of c-Myc expression by USP28.
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Objective To observe the effect of different expression levels of USP28 on the radiosensitivity of ECA109 cells by gene transfection method,aiming to provide theoretical basis for comprehensive treatment of esophageal cancer.Methods The expression levels of USP28 and c-Myc in the esophageal epithelial cells Het-1A,ECA109 and ECA109R were quantitatively measured by qRT-PCR.The specific siRNA sequences were designed according to the USP28 and c-Myc genes.The pcDNA-USP28 and pcDNA-c-Myc plasmids were constructed.The esophageal cancer cell ECA109 was transfected with Lipofectamine 2000 to observe the transfection effect and related protein expression.ECA109 and ECA109R cells were exposed to 6 Gy X-ray radiation.The cell apoptosis in each group was detected by flow cytometry.The radiosensitivity was evaluated by clone formation assay.Results The expression levels of USP28 and c-Myc in ECA109 were significantly higher than those in Het-1A (both P＜0.05),and the expression levels of USP28 and c-Myc in ECA109R were remarkably higher than those in ECA109(both P＜0.05).The pcDNA-USP28 and pcDNA-c-Myc recombinant plasmids were successfully constructed.Compared with the negative control group,the expression of USP28 at the protein and mRNA levels in the si-USP28 group was significantly down-regulated,whereas those in the pcDNA-USP28 group were remarkably up-regulated.Similar results were obtained in terms of c-Myc.Compared with the control group,the expression level of c-Myc protein was significantly up-regulated in the pcDNA-USP28 group,whereas considerably down-regulated in the si-USP28 group.After 6 Gy irradiation,the apoptosis rate and radiosensitivity of ECA109 cells were significantly declined.The apoptosis rate and radiosensitivity of ECA109R cells were increased in the si-USP28 group.Conclusions The expression of USP28 protein is closely correlated with the radiosensitivity of esophageal cancer cells.The underlying mechanism may be related to the regulation of c-Myc expression by USP28.
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Objective To examine the effect of X-ray radiotherapy on cell proliferation, migration, apoptosis, and cell cycle of human esophageal carcinoma ECA-109 cells following RNA interference (RNAi)-mediated downregulation of RNF2 gene expression.Methods The level of RNF2 mRNA expression in the human esophageal carcinoma cell line ECA-109 was determined using RT-PCR.Cell proliferation of ECA-109 was measured by MTT assay, and the changes in RNF2 protein expression in ECA-109-R cells were determined using Western blot.The changes in cell cycle and cell apoptosis at different time points following radiation were analyzed by flow cytometry, and the effect of transduction on cell migration was examined using Transwell migration assay.Data were subjected to an analysis of variance with repeated measurement design.Results The mean mRNA and protein levels of RNF2 in ECA-109 cells were significantly increased in a dose-dependent manner in the radiation group than in the control group (P0.05).The Transwell migration assay showed that the number of migrating cells following 3.5 h of radiation was significantly lower in the ECA-109-R group than in the ECA-109 and ECA-109-N groups (P<0.01).The percentage of G2/M phase cells in each group was significantly lower following 6 Gy radiation compared to that in the corresponding untreated group (P<0.01), and the percentage of G2/M phase cells was significantly lower in the ECA-109-R group than in the ECA-109 and ECA-109-N groups (P<0.05).Furthermore,cell apoptosis following radiation was also significantly higher in the ECA-109-R group than in the ECA-109 and ECA-109-N groups (P<0.01).Conclusions RNAi-mediated downregulation of RNF2 expression in esophageal carcinoma cells can reduce cell proliferation and cell migration, rescue post-radiation G2/M cell cycle arrest, promote cell apoptosis,and increase radiosensitivity.
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Objectives To explore the nutritional status of fatty acids in the newborn and their mother, and the role of the placenta in fetal nutrition. Methods The composition of fatty acids in blood, placenta, and neonatal umbilical cord blood were determined and analyzed by gas chromatography in 20 normal pregnant women. Results In 20 pregnant women in the study, average age was 27.0±4.5 years, the average gestational age of their newborns was 38.0±3.0 weeks, the average birth weight of newborns was 3320±127 g. There were 18 types of fatty acids in maternal blood, umbilical cord and placenta, including saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids. The total fatty acid content in maternal blood (3.51±0.57 g/L) was 5 times higher than that in umbilical cord blood (0.74±0.18 g/L), and there was statistically difference (P<0.05). The content of linoleic acid (LA) in maternal blood was higher than that in umbilical cord blood and placenta; the content of arachidonic acid (AA) and docosahexenoic acid (DHA) in placenta was significantly higher than that in maternal blood and umbilical cord blood; and the content of eicosapentaenoic acid (EPA) in umbilical cord blood was higher than that in maternal blood and placenta. All differences were significant (P<0.05). Conclusions Mothers preferentially transport long chain polyunsaturated fatty acids (AA and DHA) through the placenta to meet the needs of fetal and neonatal growth and development.
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Objective T o distinguish the injury characteristic changes on the drivers betw een the injuries of drivers and passengers in traffic accidents, and to provide scientific evidence for confirm ing the iden-tity of driver in traffic accidents. Methods D ata of 126 autom obile traffic accident death cases in the reclam ation areas of H eilongjiang province from 2006—2014 w ere retrospectively studied. T he injury characteristics on the drivers of autom obile traffic accident death cases w ere analyzed and the forensic identification problem in the injuries of drivers and passengers w ere discussed. Results Injuries w ere fre-quently observed on driver's neck, chest and abdom en. T he characteristic injuries caused by auto parts w ere also found, w hich appeared at the places of passenger's head, face and lim bs contacted w ith auto-m obile. Such characteristic injuries w ere not found at other places. Conclusion T he location and type of injury are associated w ith the identity of the deceased.
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Objective To explore the relationship between the levels of plasma coagulation factors (F) and acute myocardial infarction (AMI) in low age period (0.05).The multivariate analysis indicated that the FⅡ level≥ 14.27 μg/L and FⅦ level ≥22.99 μg/L were the independent risk factors for low age AMI.The value of FⅡ for diagnosing low age AMI was lower,and the optimal cut off value of Fg for diagnosing low age AMI was 22.99 μg/L,its area under ROC curve was 0.709 with a moderate diagnostic value,and the sensitivity (91.88%) and negative predictive value (86.02%) were higher,the false negative rate (13.98%) was lower,and the accuracy (70.94%) was moderate.Conclusion The FⅡ level ≥14.27 μg/L and Fg level ≥22.99 μg/L are the independent risk factors for low age AMI,and detecting the Fg level could have hint significance in diagnosing low age AMI.
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Objective T o study the m orphological characteristics of the injury caused by ox horn on hu-m an body and its injury m echanism , and to discuss the rules and characteristics of injury by ox horn and provide forensic evidences for identification of such cases. Methods T he com parative analysis of po-sition and m orphological characteristics w ere perform ed by sum m arizing the data of 100 victim s gored by ox accepted by H eilongjiang agricultural areas public security bureau during 2004-2014. Results T he specific injuries only could be found at the contact positions such as thorax and abdom en, low er back and lim bs of the victim s gored by ox horn. M ost of the skin w ounds had the characteristics of sharp in-juries, the bar-type injury by club w hich called rail w ay bruise w as an obvious characteristic appeared on the soft tissue. Conclusion O x horn can cause non-specific injuries on thorax and abdom en , low er back and lim bs of hum an body, w hich are sim ilar w ith the characteristics of sharp injury and injury by club. C areful analyzation and identification should be perform ed on such injury in daily w ork.
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Objective: To identify the relationship between the expression of protein tyrosine phosphatase non-receptor type 12 (PTPN12) and radiotherapy effect in non-small cell lung cancer (NSCLC) tissues and to determine whether PTPN12 deficiency can sensi-tize lung cancer cells to irradiation. Methods: From September 2013 to October 2014, 92 NSCLC patients undergoing radiotherapy with or without platinum-based combination chemotherapy were analyzed retrospectively. Before the treatment, PTPN12 expression was detected through immunohistochemistry. After the completion of radiotherapy, the patients' responses were assessed and radio-therapeutic efficacy analyzed. The human NSCLC cell line H1299 was infected with shPTPN12 knockdown, and colony survival assay was analyzed after irradiation. Chi-square test was used to examine the correlation between PTPN12 expression and clinicopathologi-cal characteristics. Univariate analyses and Logistic regression test were used to analyze the relationship between clinicopathological characteristics and radiotherapeutic response. Results: Patients with low PTPN12 expression were more sensitive to radiotherapy than those with high PTPN12 expression (80.0%vs. 57.1%, P=0.018). Multivariate analysis showed that PTPN12 expression was the on-ly independent predictor of radiotherapeutic response in NSCLC. The H1299-shPTPN12-knockdown cells were sensitive to irradiation. Conclusions:The results of the study indicated that downregulation of PTPN12 improved the radiosensitivity of NSCLC cells.
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Objective To investigate the effect of the novel curcumin-loaded chitosan liposomes on rabbit alkaline burns. Methods Curcumin-loaded chitosan liposomes were prepared with a film method. The particle sizes,zeta potentials and encapsula?tion efficiencies of liposomes were determined. Rabbits were randomly divided into four groups:the physiological saline group,the blank chitosan coated liposomes group,the dexamethasone group,and the curcumin-loaded chitosan liposomes group. The rabbit cor?neal alkaline burn models were built and respectively processed with the above medicines. The corneal neovascularization(CNV)and proportion of corneal epithelium healing were analyzed with the slit-lamp microscope and digital photographs. The expression of vascu?lar endothelial growth factor(VEGF)was examined with an immunohistochemical method. Results The curcumin-loaded chitosan li?posomes had the particle size of 96.6 ± 14.7 nm,with the average zeta potential of 58.8 ± 2.3 mV,and the encapsulation efficiency of 51.41±1.1%. The CNV was effectively inhibited and the expression of VEGF decreased due to the curcumin-loaded chitosan liposomes and dexamethasone. Furthermore,the curcumin-loaded chitosan liposomes improved epithelial healing of corneal more effectively than dexamethasone. Conclusion The encapsulation efficiency of the curcumin-loaded chitosan liposomes was high. The effects of the cur?cumin-loaded chitosan liposomes on inhibition of CNV and improvement of healing of cornea epithelium were obvious. Curcumin-load?ed chitosan liposomes are novel ophthalmic delivery formulations for the treatment of corneal alkaline burns.
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Background and purpose:p53 binding protein (53BP1) expresses in many normal and tumor cells. In vitroexperiments have conifrmed that inhibition of the protein expression of 53BP1 can effectively eliminate cycle arrest of tumor cells, and increase the radiosensitivity after irradiation. However, thein vivo experiment has not been re-ported. This study aimed to explore the effect of silencing 53BP1 gene on the growth and radiosensitivity to esophageal cancer cell ECA109 xenograft in nude mice.Methods:Forty-eight male BALB/c/nu nude mice were randomly divided into 6 groups: ECA109, ECA109/R, ECA109/N, ECA109/NR, ECA109/B and ECA109/BR. Three kinds of prepared cells (ECA109, ECA109/N and ECA109/B) were subcutaneously injected into the paw pads of mice (2×106/100 μL per mouse). The nude mice in ECA109/R, ECA109/NR, and ECA109/BR groups were irradiated with 15 Gy. Tumor growth was monitored every other day on the 6th day after injection. Tumor volume was measured with calipers. Theexpression levels of CHK1, CHK2 and phosphorylated CHK2-T68 protein were examined in different groups by West-ern blot. Apoptotic cell and cell cycle distribution were detected by lfow cytometry assay.Results:Visible tumors were detectable by day 7 after implantation, and the tumor volumes showed no signiifcant differences among all the groups (F=0.67, P=0.69). After irradiation with 15 Gy, tumor volumes in ECA109/BR group were smaller than those in other groups (P=0.03); the growth inhibition rate increased, but the relative growth rate decreased signiifcantly (P=0.01). The q value which relfected the radiosensitizing effect in ECA109/BR group was 1.45. The expressions of CHK1 and CHK2 at protein level in ECA109/BR group were not inlfuenced (P=0.71). However, the level of phosphorylated CHK2-T68 expression decreased signiifcantly after irradiation with 15 Gy (P=0.03). Cell cycle distribution and apoptosis were not signiifcantly different among all the groups (P=0.45).Conclusion:Silencing 53BP1 gene expression could inhibit the growth of esophageal cancer cell xenograft and increase the radiosensitivity to tumors in the nude mice.
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Transcription factor is a key trans-acting factor to mediate stress response by regulating gene expression. Plants have developed a series of mechanisms to modulate development, stress response, signaling and disease resistance at transcription level. DNA binding with one finger (DOF), containing one C₂-C₂ zinc finger domain, is a special plant transcription factor. Specifically, the conserved domain at N-terminus of DOF has multiple functions, including interacting with DNA and protein, which could be involved in plant development and stress response. Although many DOF family genes are characterized in plant stress response, it is not clear if DOF genes have functions in cereal plants. In the present paper, the role of DOF family genes on cereal plants were discussed based on a comprehensive phylogenetic relationship analysis, expression profiles in different tissues and various environmental conditions. The results obtained here will provide an important reference for further understanding the mechanism of gramineous crops in stress resistance.
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DNA-Binding Proteins , Metabolism , Gene Expression Regulation, Plant , Phylogeny , Plant Proteins , Metabolism , Plants , Genetics , Transcription Factors , Metabolism , Zinc FingersABSTRACT
[ ABSTRACT] AIM:To explore the effects of transcription factor HOXB7 silencing on the viability , invasion and migration of SGC-7901 cells.METHODS:The siRNA was designed to specifically interfere the expression of HOXB7.To assess the silence efficiency of the siRNA , the expression of HOXB7 at mRNA and protein levels was detected by real-time PCR and Western blot in the SGC-7901 cells transfected with siRNA .The cell viability was measured by MTT assay .Cell cycle-related proteins such as cyclin D 1 and CDK4 were determined by Western blot .Transwell assay was performed to an-alyze the migration ability of the SGC-7901 cells.The mRNA and protein levels of the tumor invasion-and metastasis-relat-ed molecules, such as VEGF, PTEN and p-AKT, were also detected by real-time PCR and Western blot .RESULTS:The expression of HOXB7 at mRNA and protein levels was higher in the gastric carcinoma tissues than that in the normal gastric tissues.The expression of HOXB7 at mRNA and protein levels was knockdown by siRNA .After silencing of HOXB7 gene expression, the viability of the SGC-7901 cells decreased, the expression of cyclin D1, CDK4 and VEGF was down-regula-ted, and the phosphorylation level of AKT was also obviously decreased .CONCLUSION:HOXB7 effectively promotes the viability, invasion and migration of gastric carcinoma cells by up-regulating the expression of cyclinD 1, CDK4 and VEGF, and increasing the phosphorylation level of AKT to inhibit PTEN function .
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Objective To analyze the epidemiological status and risk factors of urinary calculus in residents of zhuo-zhou city. Methods A total of 19 446 residents in zhuozhou city were surveyed with a stratified random sampling method. The questionnaire included body mass index (BMI), daily water consumption, sweets, meals taste, cooking oil, high-protein diet and family history. A 1∶1 ratio was used to select the matched normal control group. Logistic regression analysis was used to compare BMI, diet habits, family history of renal stones and other risk factors of urinary calculus between two groups. Results There were 1 752 (9.8%) patients were confirmed as urinary calculus in 17 854 follow-up members, in which there were 1 125 male and 627 female. A total of 843 patients were at 0-40 years of age (48.04%). The incidence of urinary calculus was mainly in the second and third quarter of the year, and mainly was ureteral stones. There were significant differ-ences in BMI, daily water consumption, sweets, meals taste, cooking oil, high-protein diet and family history between two groups. Logistic regression analysis showed that the protective factors included more daily water consumption, lower level of BMI and eating vegetable oil. And the risk factors of urinary calculus were salty taste, sweet taste, high protein diet and fami-ly history of urinary calculus. Conclusion Prevention measures of urinary calculus included controlling body mass, drink-ing more water, and controlling high-protein, high-sugar, high-salt food intake.
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In the process of hemodialysis, the patients' blood pressure and pulse wave are likely to change considerably so that hypotension and rapid heart rate may be dangerous to the hemodialysts' life. Based on the environment of hemodialysis room, we designed the communication system using SPCE061A single-chip computer and STC89C52 single-chip computer to realize wireless communication between upper system and the two single-chip computers, and on the upper computer the pulse wave can display at real-time and save data on the data base. At the same time we designed an interface program to reduce noise by way of frequency domain analysis and wavelet denoising. It can draw the cycle pulse wave, eigenvalue-K, the maximum peak and peak frequency, it can effectively judge a racing heart in the hemodialysis and can also evaluate vascular sclerosis of hemodialysis. In short, the system is able to improve treatment security and reduce the burden of the doctors and nurses in hemodialysis rooms.
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Humans , Algorithms , Information Systems , Monitoring, Physiologic , Methods , Pulse Wave Analysis , Radial Artery , Renal Dialysis , Software DesignABSTRACT
OBJECTIVE@#To assess how high ligation of varicocele improves sperm DNA integrity in patients with asthenospermia.@*METHODS@#The DNA fragmentation index (DFI) and semen analysis were conducted in 30 subjects with normal sperm samples, and the parameters were used as references. Fifty-six patients from the Department of Urology of the Second Affiliated Hospital of Chongqing Medical University from April 2009 to April 2011 all signed the informed consent. Semen was collected 1 week before the high ligation of varicocele and 3 months after the surgery. We compared the change of the integrity of sperm DNA and semen.@*RESULTS@#Three months after the high ligation of varicocele, DFI and semen indexes significantly improved (P0.05). Six months later, 27 spouses were pregnant out of the 53 surgical patients (50.9%). DFI of the pregnancy group and the non-pregnancy group was (13.90±9.70)% and (20.10±10.27)%, with significant different (P<0.05).@*CONCLUSION@#Varicocelectomy can improve the sperm DNA integrity in patients with asthenospermia, whose effect is not related to surgical approach, unilateral or bilateral varicocele or varicocele grade.
Subject(s)
Adult , Humans , Male , Middle Aged , Young Adult , Asthenozoospermia , General Surgery , DNA Damage , DNA Fragmentation , Ligation , Semen , Spermatic Cord , General Surgery , Spermatozoa , Pathology , Urologic Surgical Procedures, Male , Varicocele , General SurgeryABSTRACT
Objective To investigate the causes and risk factors of postpartum hemorrhage (PPH) in hepatitis B virus (HBV) infected parturient.Methods Retrospective analysis was performed on the 1021 HBV infected parturient from Shanghai Public Health Clinical Center from July 2005 to June 2011.The comparisons were done by chi-square test.Results Among 1021 cases of HBV infected parturient,868 (85.01％) were asymptomatic and the PPH rate was 2.76％ (24/868) ;the remaining 153 cases (14.99％) were chronic active hepatitis B and the PPH rate was 16.99％(26/153).The difference between two groups was statistically significant (x2 =56.541,P＜0.01).The total incidence rate of PPH was 4.89％ (50/1021) and 17 cases (34.00％) were postpartum hemorrhage＞1000mL.The causes of PPH included uterine inertia (30/50,60.00％),abnormal placenta (11/50,22.00％),dysfunction of coagulation (5/50,10.00％) and lesion of birth canal (4/50,8.00％).The risk factors of PPH included delivery mode (x2 =6.528,P=0.038),abortion times (x2 =16.269,P=0.000),delivery times (x2 =6.990,P=0.008),ALT levels (x2=56.541,P=0.000) and HBV DNA (x2 =64.706,P=0.000).Conclusions The main causes of PPH in HBV infected parturient include uterine inertia,abnormal placenta,lesion of birth canal and dysfunction of blood coagulation.PPH is correlated with abortion times,delivery times,delivery mode,liver function and HBV DNA.The incidence of PPH in parturient with chronic active hepatitis B is higher than asymptomatic parturient.
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Objective Since there are significant variation of the dietary structure recent years in China,it is necessary to re-investigate the fatty acid composition of human breast milk for the presentation of the latest data of fatty acid composition in China. Methods Using a gas chromatography GC-2010,the composition of fatty acids was detected in the human colostrums and the mature breast milk(consecutively from postnatal day 1 to day 7 and from postnatal day 42)obtained from 62 healthy postpartum women in Shanghai and Chongqing,two big cities of China,from Jan to July,2008. Results The level of total fatty acid(TFA)tended to increase significantly from the colostrums to the mature breast milk. No significant difference in the level of TFA was found between two cities. The significantly higher monounsaturates(MUFA)level(44.06% vs. 33.85%,P ＜ 0.01)and lower linoleic acid(LA,C18 : 2n-6)level(18.43% vs. 27.62%,P ＜ 0.01)of the mature breast milk were observed in Chongqing women compared with those in Shanghai women. The docosahexenoic acid(DHA)level of the mature breast milk in Shanghai women was significantly higher than that in Chongqing women(0.41% vs. 0.29%,P ＜ 0.01). There was no significant difference in the level of arachidonic acid(AA,C20 : 4n-6)between two cities. Conclusions The fatty acid composition in human breast milk tends to vary with the extension of the lactation. There is significant difference in the fatty acid compositions in human breast milk between Shanghai and Chongqing owing to different dietary habits in the different regions of China.
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Objective This paper aims at the therapeutic effect aft er the artifitial abortion by employing the TCM periodic treatment with respect to shortening the vaginal bleeding time and accelerating the uterine lining recovery. Methods Sampling 280 cases of artifitial abortion in early pregnancy, the author divides them at random the testing and control groups with each of the membership of 140 cases. Given the Nifuratel perorally same as the control group, the testing group is additionally under the TCM periodic treatment, that is, dosing Chinese medicine with the purpose of eliminating blood stasis,promoting tissue regeneration as well as invigorating the vital energy and nourishing the blood during the 1 to 3 days'postoperation period, giving Chinese medicine with a view of strenthening liver and kidney together with activating vital energy during the 4 to 14 days' postoperation period, instructing Chinese medicine in order to nourish the spleen and kidney as well as regulate Chong and Ren Vessels during the 15 to 25 days' postoperation period perorally and respectivelly. In contrast, the control group is given Xuefuzhuyu Capsules after the whole postoperation period. Results In terms of the time of vaginal bleeding, menstrual recovery and the regenerating condition of the uterine lining, the result (except for 3 cases in testing group and 2 cases in control group quiting the experiment) shows that after treatment (9 days after the abortion) the testing group achieves the effect superior to that of control group (P＜0.01). Conclusion The conclusion is that the TCM periodic therapy is effective in the aspect of shortening the vaginal bleeding time, accelerating the uterine lining recovery and regulating the menstrual circle.